Contact an Account ManagerExtraction of perfluorinated organic compounds in fish tissues
Summary
Type: SPE
Application #: 304723
Detection:
Conditions: Column conditioning: 50 mL methanol, then 10 mL water
(Do not let run the column dry.)
Sample aspiration: prepared sample is passed through the column
Washing: 2 x 20 mL water
Drying: nitrogen (approx. 1.3 h, 0.3 bar)
Elution: 50 mL methanol with 5 mM ammonium acetate and 2.5 mM acetic acid
Evaporation: to approx. 1 mL (evaporator); then with nitrogen to 800 µL (liver sample) and to 500 µL (muscle sample)
After freezing again (precipitation of lipids), 400 µL supernatant is transferred in a further vial
Authors: Theobald, N.; Gerwinski, W.; Caliebe, C.; Haarich, M.
Source: Forschungsbericht 202 22 213, UBA-FB 00 001049
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